American Airlines and JetBlue Airways announced on Monday that the two airlines will no longer sell tickets on each other's networks for select flights out of New York and Boston.
The companies will also end their frequent flyer agreement that allowed customers to earn miles or points on certain routes operated on the other airline.
The end of these agreements is effective April 1.
American and JetBlue's partnership, announced in March 2010, allowed Jet Blue customers to connect on to American's international flights out of Boston and New York's JFK Airport while American customers were able to connect on to JetBlue flights to domestic cities where the two carriers didn't compete, such as JFK to Portland, Maine or Nantucket, Mass.
The end of the partnership was expected by Wall Street analysts.
"This is not a complete surprise as we expected US Airways could provide American the feed they need in New York. More to the point, we expect New York to be less important to American going forward as US Airways has a large hub in Philadelphia," wrote Cowen and Company analyst Helane Becker in an investor note on Monday morning.